Graduateship in chemistry, College of Chemical sciences
Ph.D. in Chemistry 2001
Argonne National Laboratory, IL / Loyola University of Chicago, IL
Coordinate lectures in Biochemistry, Conductlectures in Biochemistry, Inorganic Chemistry;Labs – General,Organic, Physical Chemistry
Certified to mentor science faculty, Certified to teach Sci 220 – Human Nutrition, Bio 101- Principles of Biology, Gen 101- Skills for Lifelong Learning 1, Gen 300 – Skills for Professional Development, Gen 480 – Interdisciplinary Capstone Course, Res 110 – Introduction to Research and Information Utilization, Res 320 – Foundations of Research, Res 100 – Paradigms of Health, Sci 230 – Introduction to Life Science (Axia), Sci 256 – People, Science and the Environment, Sci 362 – Environmental Issues and Ethics
Cytokine expression as a function of Sphingomyelin levels in differentiated THP-1 cells and Macrophage. Effect of LPS, Myriocin; Growth / cell survival studies of the mutant CHO strain LY-B; LDL Oxidation by Endothelial cells determined in human vascular endothelial cells (HUVEC) and human arterial endothelial cells (HAEC); Sphingomyelin / PC ratio in raft and non raft fractions of cells grown in different growth conditions, in SPB-1 temperature sensitive CHO mutant cell line; Conjugated linoleic acids (CLA) uptake in adipocytes – raft and non raft fractions, determined by GCMS; Radioactive glucose uptake in adipocytes – effect of insulin, CLA; Synthesis of CLA
Adjunct Assistant Professor
Adapt bench-scale reactions to financially viable industrial processes, Development of bio-based catalysts and fuels, Production of acetic acid from anaerobic fermentation. Large scale production of active catalyst from microbial fermentation of Z. mobilis, extraction of periplasmic proteins, activity assays.
Advisor – Prof. Harris Ripps; Responsible for investigating gap-junction mediated cell death, Effect of taurine in the retinal pigment epithelium and electrically coupled cells in protecting against apoptotic agents utilizing molecular biology techniques, microscopy and imaging. Diabetes research – comparison of protein expression in diabetic and normal rat retinal pigment epithelium utilizing SELDI
Achievements – Research resulted in scientific publications in Apoptosis, Molecular Vision.
Paper presentation at ARVO, poster presentations; ARVO NIH Travel Grant, ASBMB Travel Grant
Responsible for teaching Biochemistry (upper division) and Human Biology (freshman); Class size-25, Lectures and labs; Designed course syllabus,Prepared handouts, lab protocols, Exams, Quizes; Ordered supplies for labs
HOX gene expression analysis in cancer cell lines, Development of constructs for RNAi in mammals and human cell lines. Down regulated upstream HOX gene products by RNAi, observed changes in expression of down- stream HOX gene products.
Construction, expression and purification of mutant reaction centers to enhance electron transfer via the naturally inactive B-branch, and decrease ET via the naturally active A-branch in R. capsulatus. Used novel detergent to purify mutant proteins in native state;
Biophysical studies to evaluate ET rates. Modified UV spectrophotometer to measure ET rates in mutant R. capsulatus RCs;Coordination of lab activities, waste management
Achievements – Graduated with a Ph.D. Laboratory Graduate Fellowship. Publication in Biochemistry
Modeling the photosynthetic reaction center of R. capsulatus
Teaching Assistant -General & Nursing Chemistry
Achievements – Graduated with a Ph.D. Graduate Student Fellowship, Loyola University of Chicago, IL.
1.Spectroscopic characterization of CuZnSOD -antioxidant enzyme. Cloned and expressed recombinant proteins. Expressed & purified yeast CuZnSOD in E. coli, Metal titrations, pH dependence, Metal substitution. Electron transfer rates as a tool to probe metal-ligand interactions, conformational changes. Collaboration project with Prof. Harry Gray’s group at Caltech, Pasadena, CA
2.Construction of Clamydomonas mutants for crystallographic studies. Transformed Clamydomonas mutant constructs by particle gun. 3.Effect of different detergents in purifying the bacterial light harvesting complex. Purified light harvesting complex for crystallographic studies. Achievements -Pre-doctoral Trainee Award, NIH Chemistry-Biology Interface Training Program, UCLA Access Fellowship
Surface analysis of bilayers;Investigated electrical conductivity of biologically active bilayers -Langmuir trough, electrochemical methods, STM -thin films on graphite.Teaching Assistant -Analytical (upper division) & General Chemistry; Taught a class of 15 students. Instrumentation – HPLC, GC, UV-Vis, NMR, IR; Troubleshooting;Achievements – Graduated with MS.
Investigation of long-range electron coupling through a hydrogen bond in model compounds. Second coordination sphere effects on electron transfer energetics. Synthesized & purified organometallic compounds, new metal adducts. Spectroscopy UV-Vis-near IR, IR, NMR; Electroanalytical methods -DPP, CV
Teaching Assistant – General, Nursing Chemistry
Achievements -Merit Fellowship, Member, Phi Lambda Upsilon -Honorary Chemical Society
Conduct lectures and labs for students in a distant education program, class size varied from 25 to over a hundred. Majority of students were employed adult learners. Prepared final exams, assignments and lab protocols; Conducted exams and quizzes; Participated in student recruitment and counseling new students. Authored and edited course material that were published.
Udawatte, C., Subbaiah, P. Conjugated linoleic acid incorporation, and its distribution in the membrane of adipocytes, work in progress
Udawatte, C., Subbaiah, P. Role of Sphingomyelin in conjugated linoleic acid incorporation, and its distribution among lipid rafts, work in progress
Udawatte, C., Subbaiah, P. Role of conjugated linoleic acids in insulin mediated and non insulin mediated glucose uptake in differentiated adipocytes, work in progress